Introduction

We adapted The MATLAB based SOFI simulation tool and modified it to more accurately represent noise, background effects, and biological patterns (mitochondria, microtubules)[1]. To account for electronic noise, a gain and standard deviation map of the sCMOS camera used in our experimental setup was generated and incorporated into the simulation .

[1] Girsault, A. et al. Sofi simulation tool: a software package for simulating and testing super-resolution optical fluctuation imaging. PLoS One 11, e0161602 (2016).